Preface vii
Acknowledgements ix
Conventions and abbreviations xi
Introduction to microtechnique 1
Fixation 12
Decalcification and other treatments for hard tissues 45
Processing and mounting 52
Dyes 74
Histological staining in one or two colours 141
Staining blood and other cell suspensions 175
Methods for connective tissue 190
Methods for nucleic acids 214
Organic functional groups and protein histochemistry 240
Carbohydrate histochemistry 274
Lipids 307
Methods for inorganic ions 337
Enzyme histochemistry: general considerations 354
Hydrolytic enzymes 361
Oxidoreductases 386
Methods for soluble organic compounds of low molecular weight 425
Metal reduction and precipitation methods 440
Immunohistochemistry 483
Miscellaneous data 520
Bibliography 534
Glossary 581
Index 587
The chemical and physical principles of fixation, staining, and histochemistry in one volume! Now in its fourth edition, Histological and Histochemical Methods has been expanded and updated with the latest techniques and developments within the field, whilst retaining the details of the classic techniques still in use. The relations of chemical structures and reactions to fixation, tissue processing, staining, enzyme location, immunohistochemistry and other procedures are explained in simple, practical terms. This book is recommended for lecturers and students in biological and biomedical disciplines using histological and histochemical techniques. Postgraduate researchers will find this to be the ideal laboratory companion, as will medical laboratory technologists preparing for their professional examinations. Every histopathology laboratory should have a copy.
